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. 2015 Nov 19;2(6):e174. doi: 10.1212/NXI.0000000000000174

Figure 1. Generation of a conditional Cxcr2 allele and genotype determination of conditional knockouts by PCR.

Figure 1

(A) Schematic diagram for Cxcr2-cKO mouse generation. (B) Schematic diagram for Cxcr2-cKO mouse genotyping. (C) Genotype determination of conditional knockouts by PCR. PCR amplification of genomic DNA using primers for the flox gene before injection of tamoxifen; the higher band (∼650 bp) indicates the wild-type allele and the lower band (∼450 bp) indicates the flox allele. PLPCreERT transgenes detection: PCR amplification of the genomic DNA using primers indicated on (B) for PLPCreERT band (∼500 bp).