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. 2015 Nov 19;2(6):e174. doi: 10.1212/NXI.0000000000000174

Figure 3. Similar myelin content in tamoxifen-treated control and Cxcr2-cKO mice.

Figure 3

(A) Brain tissues from control and Cxcr2-cKO mice 4 weeks after tamoxifen injection were stained with Black-Gold solution to analyze extent of myelination. The data shown include corpus callosum (on left) and hippocampi (on right). Scale bar: 100 μM, n = 6 mice each group. (B) Brain cells from control and Cxcr2-cKO mice (6 mice each group) 4 weeks after tamoxifen injection were isolated by Percoll gradient, then PLPeGFP positive cells were sorted. Total RNA was extracted from sorted cells, and qPCR was performed for GAPDH and PLP genes. These data represent 3 technical replicates.