Table 2.
Neutralization Activity of NBD Compounds against a Panel of HIV-1 Env Pseudoviruses
| IC50(μM) ± S.D.a | |||||||
|---|---|---|---|---|---|---|---|
| Subtype | NIH # | ENVs | NBD-556 | NBD-09027 | NBD-11021 | NBD- 11021A2 |
NBD-11021B2 |
| A | 11887 | Q259env.w6 | 6.9±0.9c | 2±0.2c | 2.8±0.1 | 1.6±0.1 | 1.3±0.4 |
| 11888 | QB726.70M.ENV.C4 | 20.2±6 | 6.3±1 | 2.6±0.1 | 1.3±0.3 | 1.5±0.6 | |
| 11890 | QF495.23M.ENV.A1 | 13.6±2.3 | 2.3±0.3 | 1.8±0.5 | 1.3±0.4 | 0.86±0.4 | |
| 11889 | QB726.70M.ENV.B3 | 11.3±2.5 | 6.1±0.3 | 1.5±0.9 | 1.3±0.4 | 2.4±0.9 | |
| 11891 | QF495.23M.ENV.A3 | 27.8±9.3 | 5.7±2.2 | 3.2±0.4 | 0.88±0.4 | 1.3±0.3 | |
| 11892 | QF495.23M.ENV.B2 | 20.4±7 | 8.6±2 | 3.7±1 | 0.6±0.4 | 1.1±0.2 | |
| BG505-T332N | 11.5±6 | 2.5±0.9 | 2.4±1 | 1±0.1 | 1.3±0.6 | ||
| KNH1144 | 20±3.2 | 8.3±0.4 | 2.1±0.1 | 2.1±0.4 | 2.3±0.2 | ||
| A/D | 11901 | QA790.204I.ENV.A4 | 12.3±1.8c | 2.4±0.4c | 3.7±0.2 | 1.4±0.05 | 1±0.3 |
| 11903 | QA790.204I.ENV.C8 | 5±1c | 3±0.3c | 2±0.1 | 1.6±0.2 | 1.6±0.2 | |
| 11904 | QA790.204I.ENV.E2 | 7.1±0.5c | 1±0.1c | 3.4±0.1 | 1.2±0.3 | 2±0.05 | |
| A2/D | 11905 | QG393.60M.ENV.A1 | 43±2 | 1.2±0.2 | 1.8±0.6 | 0.41±0.2 | 1.1±0.3 |
| 11906 | QG393.60M.ENV.B7 | 3.5±0.2 | 1.7±0.4 | 1.7±0.2 | 0.8±0.3 | 1.4±0.5 | |
| 11907 | QG393.60M.ENV.B8 | 3.6±0.1 | 1.3±0.4 | 2.6±1.4 | 0.48±0.2 | 1.5±0.6 | |
| A/E | 11603 (potential) |
CRF01_AE clone 269 | 10.7±1.5 | 3.5±1.5 | 3±0.1 | 1.5±0.3 | 1.7±0.9 |
| AA058 | 4.1 ±0.8 | 1.9±0.8 | 4±0.8 | 3.3±1.2 | 3±0.6 | ||
| CM244 | 5.6±0.8 | 3±0.5 | 2.8±0.2 | 2.8±0.3 | 2.5±0.6 | ||
| A/G | 11601 | CRF02_AG clone 263 | 7±2.2 | 2.3±0.3 | 2.2±0.3 | 1.4±0.3 | 2.2±0.7 |
| 11602 | CRF02_AG clone 266 | 4.6±1.6 | 2±0.3 | 1.6±0.5 | 1±0.2 | 1.3±0.5 | |
| 11605 | CRF02_AG clone 278 | 6.5±1.1 | 2.3±0.3 | 2.2±0.4 | 1.3±0.5 | 1.8±0.2 | |
| B | B41 | 4.5±2 | 1±0.2 | 1.1±0.1 | 0.68±0.1 | 1.1±0.4 | |
| 11563 | p1058_11.B11.1550b | 1.9±1 | 0.8±0.1 | 5.3±0.3 | 1.1±0.8 | 2.1±0.7 | |
| 11578 | pWEAUd15.410.5017b | 4.9±0.8c | 1.9±0.2c | 3±0.1 | 3±0.1 | 1.7±0.03 | |
| 11018 | QH0692, clone 42 | 2±0.6 | 1.4±0.2 | 0.7±0.2 | 0.52±0.1 | 0.9±0.3 | |
| 11022 | PVO, clone 4 | 3.5±0.7 | 1.9±0.4 | 1.7±0.1 | 1.9±0.1 | 1.1±0.2 | |
| 11023 | TRO, clone 11 | 4.1±0.6 | 2.9±0.3 | 1.3±0.2 | 1.2±0.05 | 1±0.1 | |
| 11024 | AC10.0, clone 29 | 8.9±3c | 1.4±0.3c | 1±0.2 | 0.32±0.01 | 0.74±0.25 | |
| 11033 | pWIT04160 clone 33 | 4.8±0.8 | 2.6±0.2 | 2±0.05 | 1.7±0.1 | 1±0.2 | |
| 11035 | PREJ04541 clone 67 | 3.4±0.2 | 2.1±0.1 | 1.8±0.2 | 1.6±0.3 | 1.2±0.1 | |
| 11036 | pRHPA4259 clone 7 | 4.3±0.5 | 2.5±0.2 | 2.1±0.1 | 2±0.4 | 1.2±0.06 | |
| 11037 | PTHR04156 clone 18 | 8.4±2.4 | 2.3±0.2 | 3.1±1.6 | 1±0.2 | 2±0.2 | |
| 11038 | pCAAN5342 clone A2 | 2.4±0.3c | 2.3±0.3c | 1.7±0.1 | 0.6±0.07 | 0.64±0.01 | |
| 11058 | SC422661.8 | 4.8±0.4c | 2.3±0.2c | 0.6±0.1 | 0.33±0.01 | 0.27±0.02 | |
| C | 11306 | Du156, clone 12 | 5±0.9 | 3.4±0.3 | 2.910.6 | 2.2±0.5 | 1.2±0.2 |
| 11307 | Du172, clone 17 | 4.8±0.2 | 2.4±0.3 | 1.5±0.3 | 1.9±0.5 | 1.5±0.3 | |
| 11308 | Du422, clone 1 | 6.1 ±0.5 | 4.6±0.4 | 3±0.1 | 3.3±0.4 | 2±0.6 | |
| 11309 | ZM197M.PB7 | 3.8±0.3 | 3.7±0.8 | 2±0.2 | 2.2±0.5 | 0.96±0.2 | |
| 11310 | ZM214M.PL15 | 4.1±0.7 | 3.5±0.2 | 2.6±0.1 | 2.7±0.2 | 1.7±0.6 | |
| 11311 | ZM233M.PB6 | 6.6±0.8 | 2.8±0.2 | 1.4±0.2 | 1.3±0.1 | 1.2±0.1 | |
| 11312 | ZM249M.PL1 | 6.1±0.3 | 5.7±1.3 | 3.1±0.3 | 3.1±0.5 | 1.8±0.6 | |
| 11313 | ZM53M.PB12 | 14.6±1 | 2.5±0.1 | 1.3±0.2 | 1.5±0.2 | 1.3±0.3 | |
| 11314 | ZM109F.PB4 | 6.2±0.3 | 5±0.2 | 2.2±0.4 | 3.2±0.5 | 1.9±0.3 | |
| 11315 | ZM135M.PL10a | 2.2±0.6 | 2.2±0.8 | 2.5±0.3 | 2.7±1.2 | 2.2±0.5 | |
| 11316 | CAP45.2.00.G3 | 9.6±0.3 | 7.3±1.7 | 2.1±0.1 | 1.5±0.1 | 1.2±0.1 | |
| 11317 | CAP210.2.00.E8 | 5.3±0.6 | 4.1±0.4 | 2.7±0.1 | 2.8±0.7 | 1.3±0.3 | |
| 11908 | QB099.391 M.ENV.B1 | 5.7±0.9 | 3±0.5 | 2.2±0.4 | 1.7±0.4 | 1.3±0.2 | |
| 11909 | QB099.391 M.ENV.C8 | 3.6±0.9 | 3.9±1.3 | 1.8±0.4 | 1.8±0.5 | 2.5±0.5 | |
| 11910 | QC406.70M.ENV.F3 | 3.6±0.9 | 3.9±1.3 | 1.8±0.4 | 1.8±0.5 | 2.5±0.5 | |
| D | 11911 | QA013.70I.ENV.H1 | 9.4±2.6 | 4.6±0.6 | 2.7±0.2 | 2.6±0.2 | 2.1±0.4 |
| 11912 | QA013.70I.ENV.M12 | 5.4±0.3 | 3.3±0.3 | 2.4±0.2 | 2.2±0.3 | 1.7±0.2 | |
| 11913 | QA465.59M.ENV.A1 | 4.8±0.2c | 1.5±0.2c | 3±0.3 | 0.76±0.15 | 1±0.2 | |
| 11914 | QA465.59M.ENV.D1 | 6.1±0.5 | 5.3±0.8 | 3.8±0.2 | 3.1±0.5 | 2.5±0.2 | |
| 11916 | QD435.100M.ENV.B5 | 4.4±1 | 2.9±0.5 | 2±0.6 | 1.7±0.5 | 1.7±0.6 | |
| 11918 | QD435.100M.ENV.E1 | 6.2±1.7 | 3.5±0.4 | 4±0.8 | 3.7±0.1 | 2.7±0.5 | |
| D/A | 11526 | 2.MF535.W0M.ENV.C1 | 7.2±1.5 | 10.6±0.6 | 2.7±0.3 | 1.8±0.5 | 1.7±0.8 |
| (mother) | |||||||
| 11517 (infant) |
1.BF535.W6M.ENV.A1 | 9.4±0.7 | 11±1.8 | 2.8±0.1 | 2.8±0.6 | 2.1±0.4 | |
| Control | A-MLV | >60 | >30 | 7.5±0.2 | 2.5±0.5 | 2.6±1 | |
| Control | VSV-G | >60 | >30 | 10±0.5 | 8.1±1 | 6.4±0.5 | |
| IC50(μM) Color code |
< 1 | 1-3 | >3-10 | >10 | |||
a
The reported IC50 values are means ± standard deviations (n = 3).
b
R5X4-tropic viruses; all the rest were CCR5-tropic viruses.
c
Data previously published (ref 19).