Table 3.
Primer used in real time PCR analysis
| Gene | Primer sequence |
|---|---|
| GAPDH | 5′-AACGACCCCTTCATTGAC-3′ |
| 5′-TCCACGACATACTCAGCAC-3′ | |
| PXR | 5′-GTTCAAGGGCGTCATCAACT-3′ |
| 5′-TTCTGGAAGCCACCATTAGG-3′ | |
| CYP3A11 | 5′-CTCAATGGTGTGTATATCCCC-3′ |
| 5′-CCGATGTTCTTAGACACTGCC-3′ | |
| CYP3A13 | 5′-AGCAGTGCTCTTTCCTTTGC-3′ |
| 5′-CCCTTTGGGAATGAATAGCC-3′ | |
| MDR1α | 5′-GTCCCAACTGGGATATTGTACA-3′ |
| 5′-AGGTGCCCATGTCTGAGTAA-3′ | |
| GSTα1 | 5′-CCCCTTTCCCTCTGCTGAAG-3′ |
| 5′-TGCAGCTTCACTGAATCTTGAAAG-3′ | |
| TNF-α | 5′-GGCAGGTCTACTTTGGAGTCATTGC-3′ |
| 5′-ACATTCGAGGCTCCAGTGAATTCGG-3′ | |
| IL-6 | 5′-CTGGTGACAACCACGGCCTTCCCTA-3′ |
| 5′-ATGCTTAGGCATAACGCACTAGGTT-3′ | |
| iNOS | 5′-CCTTGTTCAGCTACGCCTTC-3′ |
| 5′-AAGGCCAAACACAGCATACC-3′ | |
| MCP | 5′-CCCCAGTCACCTGCTGTTAT-3′ |
| 5′-TCCTGAACCCACTTCTGCTT-3′ |
Abbreviations: CYP, cytochrome P450; GSTα1, glutathione S-transferase alpha 1; IL-6, interleukin 6; iNOS, inducible nitrogen oxide synthase; MDR1α, multidrug resistance 1 alpha; PXR, pregnane X receptor; TNF-α, tumor necrosis factor-alpha; MCP, monocyte chemoattractant protein.