Table 2. Plasmids.
| Plasmid | Genotype |
|---|---|
| pACT2a | 2µ GAL-AD LEU2 |
| pRK329 | 2µ GAL-BD TRP1 |
| pRK505 | CEN/ARS GAL1p-STE6myc TRP1 |
| pRK581 | 2µ GAL-BD-MOS10/VPS60 TRP1 |
| pRK642 | 2µ GAL-BD-VPS4 TRP1 |
| pRK770 | 2µ SNF7-GFP URA3 |
| pRK842 | 2µ GAL-BD-VPS20 TRP1 |
| pRK942 | 2µ GAL-BD-SNF7 TRP1 |
| pRK1157 | 2µ CHM7-GFP URA3 |
| pRK1216 | 2µ GAL-AD-CHM7 LEU2 |
| pRK1263 | 2µ HXT7p-mCherry-CHM7 TRP1 |
| pRK1266 | 2µ CHM7-mCherry URA3 |
| pRK1332 | 2µ HXT7p-yEGFP-CHM7 TRP1 |
| pRK1405 | CEN/ARS HXT7p-yEGFP-CPS1 TRP1 |
| pRK1436 | 2µ CHM7∆MIM1-mCherry (aa 414–421 deleted) URA3 |
| pRK1464 | CEN/ARS CHM7-3HA URA3 |
| pRK1511 | CEN/ARS CHM7∆MIM1 (aa 414–421 deleted) ADH1t TRP1 |
| pRK1514 | 2µ HMG1 URA3 |
| pRK1516 | CEN/ARS CHM7 ADH1t TRP1 |
| pRK1531 | 2µ GAL-BD-IST1 TRP1 |
| pRK1532 | 2µ GAL-BD-DID2 TRP1 |
| pRK1533 | 2µ GAL-BD-DID4/VPS2 TRP1 |
| pRK1534 | 2µ GAL-BD-VPS24 TRP1 |
| pRK1536 | 2µ GAL-BD-VTA1 TRP1 |
| pRK1651 | 2µ-DID2-mCherry URA3 |
| pRK1652 | 2µ-IST1-mCherry URA3 |
| pRK1653 | 2µ-MOS10-mCherry URA3 |
| pRK1654 | 2µ-SNF7-mCherry URA3 |
| pRK1655 | 2µ-VPS2-mCherry URA3 |
| pRK1656 | 2µ-VPS20-mCherry URA3 |
| pRK1657 | 2µ-VPS24-mCherry URA3 |
| pRK1659 | 2µ-HMG1 TRP1 |
a
Clontech, Palo Alto, CA.