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. 2015 Oct 26;201(4):1427–1438. doi: 10.1534/genetics.115.180919

Figure 2.

Figure 2

An rlm1Δ mutant affects IME1 and IME2 expression and is suppressed by IME1 overexpression. (A) Effect of rlm1Δ on IME expression in IME+ colonies. ime1Δ-lacZ/IME1+ and ime2Δ-GFP/IME2+ expression in 6-day spot colonies that are either RLM1+ (wild type = solid bars, SH3827 and SH5105, respectively) or rlm1Δ (hatched bars, SH4799 and SH4789, respectively) (n = 4). (B) Effect of rlm1Δ on IME expression in ime1Δ colonies. ime1Δ-lacZ/ime1Δ and ime2Δ-GFP/ime2Δ expression in 6-day spot colonies that are either RLM1+ (wild type = solid bars, SH3830 and SH5071, respectively) or rlm1Δ (hatched bars, SH4800 and SH5072, respectively) (n =4). (C) Effect of IME1 overexpression on ime2-GFP expression in rlm1Δ colonies. GFP expression levels in RLM1 ime2-GFP and rlm1Δ ime2-GFP spot colonies containing only the control vector (left, SH 5280 and SH 5276) or containing a high-copy IME1 plasmid (right, SH5282 and SH5278) (n = 4). (D) Effect of IME1 overexpression on spore formation in rlm1Δ colonies. RLM1+ and rlm1Δ spot colonies contain only the control vector (SH5074 and SH5094) or contain a high-copy IME1 plasmid (SH5073 and SH5085) (n = 3).