Table 1. Colony partitioning and Rlm1 dependence in suboptimal sporulation environments.
Condition | Spore formation (%)c | |||||
---|---|---|---|---|---|---|
% KOAc | Temp. (°C) | % Permeablea | % lacZ+b | Wild type | rlm1Δ | Wild type/rlm1Δ |
2 | 30° | 31 ± 1 | 6 ± 0.2 | 50 ± 0.2 | 35 ± 2 | 1.4 |
0.5 | 30° | 66 ± 1 | 30 ± 2 | 24 ± 2 | 5 ± 2 | 4.8 |
2 | 34° | 48 ± 1 | 31 ± 1 | 31 ± 2 | 10 ± 1 | 3.1 |
Percentage of cells in colony permeable to PI after suspension in 2 M sorbitol (n = 3).
Percentage of cells in UASRlm1-lacZ colony producing visible level of X-gal metabolite (n = 3).
Percentage of cells in colony that have formed spores (n = 3).