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. 2015 Oct 19;201(4):1511–1523. doi: 10.1534/genetics.115.180737

Figure 6.

Figure 6

Cbl regulates endocycle through CTPsyn. (A–B′′) Merged surface confocal sections of Flag-CTPsyn-overexpressing egg chambers were stained with anti-Flag (A′ and B′, green) and anti-CTPsyn (A′′ and B′′, red) antibodies. (C–D′′) Multiple merged-surface confocal sections of Flag-CTPsyn-overexpressing egg chambers were stained with anti-Flag (green) antibody. The Flag-CTPsyn signal is shown in Cbl+/+or Cbl-/+cells (Myc-positive) and Cbl−/− follicular cells (Myc-negative). (E–G′′) BrdU incorporation is shown in Cbl mosaic stage-9 egg chambers with or without Flag-CTPsyn transgene expression. Cbl−/− clone cells are marked by the absence of GFP (outlined in white), while Cbl+/+ twin clone cells are marked by strong GFP-positive staining (outlined in yellow). BrdU signal is shown in red. DAPI-stained nuclei (blue). Bar: 20 μm (A–D) and 10 μm (E–G).