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. 2015 Jun 29;213(1):112–121. doi: 10.1093/infdis/jiv354

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© The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

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Figure 2. — Reverse transcription–polymerase chain reaction (PCR) detection of LRV1 in Leishmania braziliensis. Agarose gel electrophoresis of PCR products obtained using the LRV universal primers SMB4647 and SMB4648 with randomly primed complementary DNA derived from RNA from the species/strains is shown, as described in “Materials and Methods” section. M, double-stranded DNA (dsDNA) molecular weight marker (1 kb plus; Life Technologies, CA). Lanes 1–11: L. braziliensis isolates LC2143 (lane 1), LC2147 (lane 2), LC2176 (lane 3), LC2177 (lane 4), LC2284 (lane 5), LC2289 (lane 6), LC2321 (lane 7), LC2353 (lane 8), LC2367 (lane 9), LC2398 (lane 10), and LC2318 (lane 11). Lanes 12 and 13, L. guyanensis isolates: Lg17 (LRV1 negative; lane 12) and Lg5313 (LRV1 positive; lane 13).