Table 3.
Statistical characteristics of clone libraries of 16S rRNA gene sequences for root- and tuber-associated bacteria of 8 potato genotypes.
| Statistics and diversity indexes | Clone libraries | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||||||||
| Root | Tuber | |||||||||||||||
|
|
|
|||||||||||||||
| R1a | R2 | R3 | M1 | M2 | S1 | S2 | S3 | R1 | R2 | R3 | M1 | M2 | S1 | S2 | S3 | |
| No. of sequences | 156 | 165 | 173 | 162 | 170 | 175 | 164 | 177 | 135 | 53 | 43 | 97 | 69 | 92 | 81 | 95 |
| No. of singletons | 50 | 47 | 43 | 44 | 40 | 60 | 68 | 51 | 20 | 17 | 10 | 31 | 21 | 25 | 16 | 18 |
| Library coverage (%)b | 67.9 | 71.5 | 75.1 | 72.8 | 76.5 | 65.7 | 58.5 | 71.2 | 85.2 | 67.9 | 76.7 | 68.0 | 69.6 | 72.8 | 80.2 | 81.1 |
| No. of OTUsc | 71 | 75 | 70 | 71 | 74 | 86 | 93 | 81 | 27 | 22 | 14 | 41 | 31 | 32 | 24 | 29 |
| Chao1 | 224.1 | 152.2 | 152.1 | 138.6 | 119.9 | 246.9 | 244.9 | 187.3 | 65.0 | 67.3 | 29.0 | 118.5 | 73.0 | 182.0 | 54.0 | 59.6 |
| ACE | 456.2 | 264.4 | 245.7 | 231.5 | 175.6 | 438.3 | 541.7 | 306.1 | 198.4 | 382.7 | 36.5 | 301.6 | 107.1 | 300.1 | 105.7 | 140.3 |
| Shannon (H′) | 3.8 | 3.9 | 3.8 | 3.8 | 4.0 | 4.0 | 4.2 | 4.0 | 1.8 | 2.5 | 1.7 | 2.9 | 2.9 | 2.6 | 2.2 | 2.6 |
| Simpson (1/D) | 31.5 | 31.4 | 34.1 | 32.3 | 47.3 | 40.2 | 56.2 | 46.1 | 3.5 | 8.2 | 2.7 | 7.2 | 11.1 | 7.5 | 4.1 | 8.5 |
a
R, M, and S indicate the phenotypes of the 8 potato genotypes: resistant, medium resistant, and susceptible to potato scab diseases, respectively. R1, R2, R3, M1, M2, S1, S2, and S3 are designated for clone libraries of the 8 potato genotypes (Yukirasha, 02005-10, Snow March, Star Ruby, Snowden, Irish Cobbler, Toyoshiro, and Piruka, respectively).
b
CX=1 (n/N), where nx is the number of singletons that are encountered only once in a library and N is the total number of clones.
c
OTUs were defined at ≥97% sequence identity.