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. 2015 Dec 9;30(4):321–329. doi: 10.1264/jsme2.ME15153

Fig. 2.

Fig. 2

Molecular phylogeny of the gut symbiotic bacteria of Physopelta species. The tree displays a maximum likelihood (ML) phylogeny of eight OTUs of the gut symbiotic bacteria identified from Physopelta gutta, P. parviceps and P. slanbuschii together with selected representatives of the different Burkholderia groups. The alignment of 1,356 nucleotide sites of the bacterial 16S rRNA gene was used. The gut symbionts of the Physopelta species are shown in bold. The origins or sources of isolation of the Burkholderia strains/sequences are represented in parentheses. Accession numbers in the DNA database (DDBJ/EMBL/GenBank) are shown in square brackets. Stars indicate gut symbionts detected from the oriental chinch bug Cavelerius saccharivorus (Lygaeoidea: Blissidae) in a previous study (31). The major Burkholderia clades (BCC&P, SBE, and PBE) and the subclade “insect-associated PBE (iPBE)” are indicated on the right. Bootstrap values higher than 50% are depicted at the nodes. A phylogeny of all of the 161 Burkholderia sequences obtained is shown in Fig. S1. ML and neighbor-joining (NJ) analyses gave essentially the same results (see Fig. S2).