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. 2015 Dec 11;10(12):e0144541. doi: 10.1371/journal.pone.0144541

Fig 3. Medicarpin heals cortical bone defects by activating Wnt canonical signaling pathway.

Fig 3

(A) qPCR analysis of Wnt signaling components like β-catenin, GSK-3β, Dvl, Fzd and Lrp5. (B) Western blot analysis and densitometric analyses of phospho and non-phospho β-catenin, GSK-3β and LEF-1. (C) Med treatment to Ovx rats promotes immunohistochemical localization of β-catenin in the area surrounding the drill hole injury area compared to Sham and Ovx control groups and was equivalent to PTH treatment group. Data represent three independent experiments and expressed as mean ± SEM with 95% confidence interval. Statistical analysis was performed by ANOVA method followed by the Newman–Keuls test of significance using Prism version 3.0 software. ***P< 0.001 compared with Ovx + vehicle group; **P< 0.01compared with Ovx + vehicle group; *P< 0.05compared with Ovx + vehicle group.