Motility was measured as in Fig 1 in individual males (A) and females (B). White bars show the significant increase in worm motility in response to 10 μM capsaicin in the absence of SB 366791. Differently patterned bars designate worm motility in 0 vs. 10 μM capsaicin following pre-incubation in (and co-exposure to) SB 366791 at concentrations of 0.4 μM (horizontal lines), 0.5 μM (checkerboard), 1 μM (diagonal lines), 2 μM (squares) or 10 μM (vertical lines). The pair of black bars shows responses of worms to 100 μM capsaicin in the presence of 100 μM SB 366791 (see Methods for details). There is a significant increase in worm motility in response to capsaicin only in the absence of SB 366791. *, **, ***, P<0.05, P<0.01, P<0.001 respectively, paired t-tests vs. no-capsaicin controls. n = 4–8.