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. 2015 Dec 11;11(12):e1005731. doi: 10.1371/journal.pgen.1005731

Fig 3. daf-12/NHR and dbl-1/TGF-β signaling pathways mediate cell-nonautonomous effects of daf-16 on developmental arrest.

Fig 3

(A-C) The proportion of larvae with at least one M lineage division after seven days of starvation is plotted for various genotypes and treatments. (B) Dimethyl sulfoxide (DMSO; solvent), dafachronic acid (DA). (D) DIC merged with GFP of daf-16; daf-36 and daf-16; dbl-1 with the Phlh-8::GFP reporter after seven days of starvation. Only one M lineage cell is visible in each case; compare Fig 1C. Scale bar: 20μm (E) The average number of divided seam cells after three days of starvation is plotted. (A-C, E) Error bars reflect SEM of three-seven biological replicates. *p<0.05; **p≤0.01; unpaired t-test against daf-16. (F) DIC merged with GFP and GFP alone of daf-16; daf-36 and daf-16; dbl-1 with AJM-1::GFP after three days of starvation. Arrowheads point to seam cells that have recently divided. Compare Figs 1G and S3. Scale bar: 20μm.