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Raw (a) and time-gated (b) images of a rat mast cell fluorescently labeled with YFP-tubulin and quantum dot labeled IgE-FcεRI. Reduction of the shorter-lived YFP fluorescence by the time gate results in a more of punctate appearance of the longer-lived QD-IgE-FcεRI emission and improved particle tracking. Reproduced with permission from DeVore et al., Proc. SPIE 9338, 933812 (2015). Copyright 2015 SPIE.
