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. 2015 Dec 12;15:116. doi: 10.1186/s12935-015-0269-y

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© Yu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of hypoxia on expression levels of HIF-1α, P-gp, MRP1 and LRP protein in HBx-HepG2 cells. Western blot assay was conducted on HBX-HepG2 cells pre-incubated for 48 h in normoxic (21 % O₂) or hypoxic (5 % O₂, 3 % O₂, or 1 % O₂) conditions to measure the expression levels of HIF-1α, P-gp, MRP1 and LRP protein, band densities were normalized to β-actin. All data were expressed as mean ± s.e.m., n = 4; significant differences between hypoxia (1 % O₂) group and normoxia group were indicated as *P < 0.05, **P < 0.01; significantly differences between hypoxia (3 % O₂) group and normoxia group were indicated as ^# P < 0.05 (One-way ANOVA followed by Bonferroni’s multiple comparison tests)