Fig. 5.
Effect of B. bifidum S17/pMgapS6C and CB1954 on HT-29/EGFP4 MCTS. a Vector map of pMgapS6C for expression of a secreted chromate reductase in bifidobacteria. b Chromate reductase activity in the supernatants of B. bifidum S17/pMgapS6C (S17/ChrR6) or the empty vector control strain B. bifidum S17/pMDY23-Pgap (S17/-). c HT-29/EGFP4 MCTS were incubated with S17/ChrR6 alone or in combination with 100 µM CB1954 (CB) for up to 96 h. As controls, MCTS were treated with 200 nM staurosporine (stauro), CB1954 (CB) or medium alone (-). Fluorescence intensities were normalized to give 100 % for each MCTS at the start of the experiment (t = 0 h). Values are relative fluorescent units (RFU) per MCTS and are mean ± SEM of three MCTS. Results of one representative of three independent experiments are shown. Statistical analysis was performed using two-way ANOVA with Bonferroni post-test correction for multiple comparisons (***difference to untreated MCTS significant with adjusted P < 0.001, all other comparisons not significant)