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. 2015 May 14;17(11):4379–4393. doi: 10.1111/1462-2920.12863

Figure 1.

Figure 1

Schematic representation of the Tn5 mutants screening procedure in multi-host system.A. The whole procedure consisted of the following steps, with the remaining number of candidates shown after each step: size of transposon library tested (57 360 Tn5 mutants), primary screening (404 Tn5 mutants selected from in vitro screening and 275 from C. elegans and D. melanogaster screening), secondary screening (28 Tn5 mutants selected for cell culture models). The final 8 candidates were tested for virulence attenuation in a mouse pneumonia model.B. Summary of the results of the in vitro screening. A total of 404 Tn5 mutants having pleiotropic phenotypes (impaired in protease, pyocyanin and swarming motility) were selected from the primary screening and further tested in C. elegans and D. melanogaster disease models (C).D. Summary of the results of the transposon library.