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Invasion and IL-8 release in A549 cells of selected Tn-5 mutants.(A) Invasion by antibiotic exclusion assay and (B) IL-8 release by ELISA were determined in A549 cells after infection with wild-type and Tn5 mutants. Data, from three independent experiments, are expressed as mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test.
