Figure 3.

In vitro phenotypic characterization of P. aeruginosa PAO1-L and selected targets. Eight Tn5 mutants (kdpB, PA2414, pvdQ, PA3613, bphO, PA4916, PA5156, crc) were selected for further characterization.A. Protease, elastase, pyocyanin and pyoverdine production was determined in supernatant from culture grown to stationary phase using colorimetric assays. For twitching motility, cells were inoculated with a toothpick from an LB agar plate onto a twitching plate (tryptone broth plus 1% agar). The diameter of the twitching zone was determined. Values of triplicate cultures are given.B. Biofilm formation was assessed using a bioflux microfluidic channel. Biofilm cells were continuously grown in 10% LB at 37°C for a period of 14 h. Left panel shows a confocal microscope picture of the biofilms and right panel the representation of the surface coverage for each mutant. Standard deviations are based on the mean values of six images taken in random locations in the microfluidic channel.C. For swarming and swimming motility, cells were inoculated from a 16 h LB culture onto swarming or swimming plates containing 0.5% or 0.3% agar respectively.