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Biochemical properties of FRET-based ABA reporters
| ABA reporter | ABA receptor |
K’d [µM] |
In vitro DR |
|---|---|---|---|
| ABACUS1-2 µ | PYL1 H87P | 2 | 1.6 |
| ABACUS1-80 µ | PYL1 | 80 | 2.6 |
| ABAleon2.1 | PYR1 | 0.1 | 1.1 |
| ABAleon2.15 | PYR1 H115A | 0.5 | 1.1 |
Indicated are the reporter names, the ABA receptor incorporated in the sensory module, their apparent ABA affinity (K’d) and in vitro dynamic range (DR) calculated from ABA-bound emission ratio/ ABA-unbound emission ratio.
