FIGURE 4.

Analysis of surfactant capability of each Polar supernatant on S. epidermidis O-47. The center of each well of a 24-well tissue-culture-treated polystyrene microtiter plate was coated with each supernatant. After evaporation, the wells were then filled with staphylococci and incubated at 37°C. Then wells were rinsed with water and stained with 1 ml of 0.1% crystal violet. Stained biofilms were rinsed with water and dried, and the wells were photographed. (A) Supernatants derived from planktonic growths. (B) Supernatants derived from biofilm growths.