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. 2015 Dec 14;2:76. doi: 10.3389/fvets.2015.00076

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Copyright © 2015 Uddin, Suen, Prow, Hall and Bielefeldt-Ohmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of viral RNA expression in in vitro WNV-infected PBMCs. WNV_NSW2011 RNA expression in in vitro virus-infected PBMCs at different time points was been quantified using qRT-PCR. The ΔΔCt (ΔΔCt = ΔCt_WNV − ΔCt_mock) values were calculated by subtracting the ΔCt of genes in mock-inoculated PBMCs (n = 3). Ct for the mock-inoculated PBMCs (undetected) was set to 40. The bar graph showed the expression of genes in WNV-infected PBMCs over mock-inoculated PBMCs (fold change: the normalized expression value of a gene in WNV-stimulated cells/the normalized expression value of a gene in mock-inoculated cells).