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. 2015 Dec 14;2:76. doi: 10.3389/fvets.2015.00076

Table 1.

List of primer sequences used in this study.

Gene Primer seta Amplicon size (bp) GenBank accession number
Kunjin F: AACCCCAGTGGAGAAGTGGAb 70 D00246
R: TCAGGCTGCCACACCAAA
IFNA F: TGCTTGCAGGACAGACATGA 95 XM_002708065.1
R: ATCTCGTGGAGCACAGAGAT
IFNB F:TCCAACTATGGCACGGAAGTCTc 89 XM_002707968
R:TTCTGGAGCTGTTGTGGTTCCT
IFNG F:TGCCAGGACACACTAACCAGAGc 127 NM_001081991
R:TGTCACTCTCCTCTTTCCAATTCC
TNFA F:CTGCACTTCAGGGTGATCGc 94 NM_001082263
R:CTACGTGGGCTAGAGGCTTG
IL6 F: CTACCGCTTTCCCCACTTCAGc 135 NM_001082064.2
R: TCCTCAGCTCCTTGATGGTCTC
IL12/IL23P40 PS211: CTCCGAAGAAGATGGCATTACCc 126 XM_002710347
PS212: TCTCCTTTGTGGCAGGTGTATTG
IL22 PS567: ACCTCACCTTCATGCTGGCTAA 84 XM_002711248
PS568: CATGGAACAGCTCATTCCCAAT
CXCL10 F: ATAGAAGCATCCTGAGCCCA 86 XM_002717106.1
R: GAACTGCAAACTGAGGCCAA
PTX3 F: TTCCCCATGCGTTCCAAGAA 95 XM_002716328.1
R: GTGGCTTTGACCCAAATGCA
HO1 F: ACTGCCGAGGGTTTTAAGCT 88 XM_002711415.1
R: GGTTCTCCTTGTTGTGCTCA
iNOS F: GACGTCCAGCGCTACAATATCCc 102 XM_002718780
R: GATCTCTGTGACGGCCTGATCT
Caspase 3 F: AAGCCGACTTCCTGTATGCA 111 NM_001082117.1
R: CGTACTCTTTCAGCATGGCA
Caspase 9 F: AAACGTGGATTTGGCGTACG 80 XM_002722329.1
R: TGCTGCTGAAGTTCACGTTG
TLR1 F: TGTGTCCCACAATGAGCTGT 93 XM_002709270.1
R: GGCAGAGCATCAAACGCATT
TLR2 F: GCTGCGCAAGATCATGAACA 96 NM_001082781.1
R: TTTATGGCGGCCCTCAAGTT
TLR3 F: ATGACCTGCCCACCAACATA 140 NM_001082219.1
R: TTCTGGCTCCAGCTTTGAGA
TLR4 F: AGGCTGTTGGTGGAAGTTGA 91 NM_001082732.2
R: TGCTTATCTGACAGGTGGCA
TLR6 F: CATTGAGCACAACGCAGTGT 108 XM_002709388.1
R: AGCTCGCATGTACAGTGGAA
TLR10 F: ACACCGGTAATGCACTTGGA 85 XM_002709387.1
R: TAAGCAAGGTGTCTGGCCAT
MyD88 F: GCCAGTGAGCTCATCGAGAA 80 XM_002723869.1
R: TCACACTCCTTGCTCTGCAG
IRF1 F: AGCACTGTCACCACATAGCA 120 NM_001171347.1
R: TCATCTGTCGCAGCTTCAGA
IRF7 F: AAGTGCAAGGTGTACTGGGA 119 XM_002724304.1
R: AGCTCTTGGAAGAAGGTGCT
IRF9 F: TAACTGAGGCTGCTGTGCAA 103 XM_002718097.1
R: ACACGCCCGTTGTAGATGAA
TRAF3 F: TGGCTATAAGATGTGCGCCA 95 XM_002721716.1
R: ACTCTCCACGCATGATGACA
STAT1 F: TTCAACATCCTGGGCACACA 112 XM_002712346.1
R: TGCCAGCGTTCTTCTGTTCT
GAPDH F: TGACGACATCAAGAAGGTGGTG** 126 NM_001082253
R: GAAGGTGGAGGAGTGGGTGTC
PPIA F: AGGGCATGAGCATTGTGGAA 86 NM_001082057.1
R: TCCACAGTTGGCAATGGTGA

aAnnealing temperature was 60°C for all the primer sets.

bPrimer set is adopted from Ref. (34).

cPrimer set are adopted from Ref. (35).