Table 1.
Gene | Primer seta | Amplicon size (bp) | GenBank accession number |
---|---|---|---|
Kunjin | F: AACCCCAGTGGAGAAGTGGAb | 70 | D00246 |
R: TCAGGCTGCCACACCAAA | |||
IFNA | F: TGCTTGCAGGACAGACATGA | 95 | XM_002708065.1 |
R: ATCTCGTGGAGCACAGAGAT | |||
IFNB | F:TCCAACTATGGCACGGAAGTCTc | 89 | XM_002707968 |
R:TTCTGGAGCTGTTGTGGTTCCT | |||
IFNG | F:TGCCAGGACACACTAACCAGAGc | 127 | NM_001081991 |
R:TGTCACTCTCCTCTTTCCAATTCC | |||
TNFA | F:CTGCACTTCAGGGTGATCGc | 94 | NM_001082263 |
R:CTACGTGGGCTAGAGGCTTG | |||
IL6 | F: CTACCGCTTTCCCCACTTCAGc | 135 | NM_001082064.2 |
R: TCCTCAGCTCCTTGATGGTCTC | |||
IL12/IL23P40 | PS211: CTCCGAAGAAGATGGCATTACCc | 126 | XM_002710347 |
PS212: TCTCCTTTGTGGCAGGTGTATTG | |||
IL22 | PS567: ACCTCACCTTCATGCTGGCTAA | 84 | XM_002711248 |
PS568: CATGGAACAGCTCATTCCCAAT | |||
CXCL10 | F: ATAGAAGCATCCTGAGCCCA | 86 | XM_002717106.1 |
R: GAACTGCAAACTGAGGCCAA | |||
PTX3 | F: TTCCCCATGCGTTCCAAGAA | 95 | XM_002716328.1 |
R: GTGGCTTTGACCCAAATGCA | |||
HO1 | F: ACTGCCGAGGGTTTTAAGCT | 88 | XM_002711415.1 |
R: GGTTCTCCTTGTTGTGCTCA | |||
iNOS | F: GACGTCCAGCGCTACAATATCCc | 102 | XM_002718780 |
R: GATCTCTGTGACGGCCTGATCT | |||
Caspase 3 | F: AAGCCGACTTCCTGTATGCA | 111 | NM_001082117.1 |
R: CGTACTCTTTCAGCATGGCA | |||
Caspase 9 | F: AAACGTGGATTTGGCGTACG | 80 | XM_002722329.1 |
R: TGCTGCTGAAGTTCACGTTG | |||
TLR1 | F: TGTGTCCCACAATGAGCTGT | 93 | XM_002709270.1 |
R: GGCAGAGCATCAAACGCATT | |||
TLR2 | F: GCTGCGCAAGATCATGAACA | 96 | NM_001082781.1 |
R: TTTATGGCGGCCCTCAAGTT | |||
TLR3 | F: ATGACCTGCCCACCAACATA | 140 | NM_001082219.1 |
R: TTCTGGCTCCAGCTTTGAGA | |||
TLR4 | F: AGGCTGTTGGTGGAAGTTGA | 91 | NM_001082732.2 |
R: TGCTTATCTGACAGGTGGCA | |||
TLR6 | F: CATTGAGCACAACGCAGTGT | 108 | XM_002709388.1 |
R: AGCTCGCATGTACAGTGGAA | |||
TLR10 | F: ACACCGGTAATGCACTTGGA | 85 | XM_002709387.1 |
R: TAAGCAAGGTGTCTGGCCAT | |||
MyD88 | F: GCCAGTGAGCTCATCGAGAA | 80 | XM_002723869.1 |
R: TCACACTCCTTGCTCTGCAG | |||
IRF1 | F: AGCACTGTCACCACATAGCA | 120 | NM_001171347.1 |
R: TCATCTGTCGCAGCTTCAGA | |||
IRF7 | F: AAGTGCAAGGTGTACTGGGA | 119 | XM_002724304.1 |
R: AGCTCTTGGAAGAAGGTGCT | |||
IRF9 | F: TAACTGAGGCTGCTGTGCAA | 103 | XM_002718097.1 |
R: ACACGCCCGTTGTAGATGAA | |||
TRAF3 | F: TGGCTATAAGATGTGCGCCA | 95 | XM_002721716.1 |
R: ACTCTCCACGCATGATGACA | |||
STAT1 | F: TTCAACATCCTGGGCACACA | 112 | XM_002712346.1 |
R: TGCCAGCGTTCTTCTGTTCT | |||
GAPDH | F: TGACGACATCAAGAAGGTGGTG** | 126 | NM_001082253 |
R: GAAGGTGGAGGAGTGGGTGTC | |||
PPIA | F: AGGGCATGAGCATTGTGGAA | 86 | NM_001082057.1 |
R: TCCACAGTTGGCAATGGTGA |