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. 2015 Sep 2;41(1):45–57. doi: 10.1038/npp.2015.224

Figure 2.

Figure 2

Pre-stressor administration of metyrapone attenuates SEFL, and co-administration of metyrapone and CORT restores SEFL. (a) Experimental design for metyrapone administration. (b) Plasma CORT levels (ng/ml; mean+SEM) after the 15-shock trauma on Day 1. Main effect of stress, p<0.001; linear trend analysis for stressed animals, p<0.05 (two-way ANOVA, followed by planned contrasts). (c) The mean+SEM of freezing percentage in stress context test on Day 4. Drug × stress, p<0.0001 (two-way ANOVA), linear trend analysis for stressed animals, p<0.0001. (d) The mean+SEM of freezing percentage during the generalization test in the conditioning context on Day 5. No significant differences were found for any main effect or interaction (two-way ANOVA). (e) Freezing (mean+SEM) in conditioning context on Day 7. Drug × stress interaction, p<0.05, linear trend analysis for stressed animals, p<0.0005 (two-way ANOVA, followed by planned contrasts). (f) Experimental design for metyrapone and CORT co-administration. (g) Freezing (mean+SEM) in the stress context on Day 4. First digit in group designations=metyrapone dose (0 or 150 mg/kg); second=CORT dose (0 or 10 mg/kg). ***p<0.0001 (overall one-way ANOVA, followed by planned contrasts). (h) Freezing (mean+SEM) in conditioning context on Day 7. First digit in group designations=metyrapone dose (0 or 150 mg/kg); second=CORT dose (0 or 10 mg/kg). **p<0.01; ***p<0.0001 (overall one-way ANOVA, followed by planned contrasts).