Skip to main content
. 2015 Dec 10;41(1):374–376. doi: 10.1038/npp.2015.262

Figure 1.

Figure 1

Examples of in vitro RiboTag applications for primary cultures. (a) Representation of translating RNA isolation using RiboTag immunoprecipitation. (b) Cell-type specific: cultures containing mixed cellular populations are transfected with RiboTag plasmids that restrict RiboTag expression to specific cells using cell type-specific promoters. RiboTag-isolated RNA from specific cell types can then be quantified. (c) Transgenic manipulation: cell cultures are transfected with RiboTag alone or co-transfected with transgenes that enhance or inhibit expression of specific proteins. RiboTag-isolated RNA can measure the effect of transgenic manipulations on RNA translation. (d) Pharmacological manipulation: cultures are transfected with RiboTag and then treated with drug or vehicle. Drug-induced changes in RNA translation can then be measured using RiboTag-isolated RNA from vehicle and drug-treated samples.