Table 1.

Primer set for V3 16S rDNA sequencing and PCR/real time RT-PCR tests targeting the classical and newly described staphylococcal enterotoxin genes.

	Primers set and sequences (5′-3′)	Gene	Amplicon (bp)	References
SEAf	TTGGAAACGGTTAAAACGAA	sea	120	[17]
SEAr	GAACCTTCCCATCAAAAACA
SEBf	TCGCATCAAACTGACAAACG	seb	478	[17]
SEBr	GCAGGTACTCTATAAGTGCC
SECf	GACATAAAAGCTAGGAATTT	sec	257	[17]
SECr	AAATCGGATTAACATTATCC
SEDf	CTAGTTTGGTAATATCTCCT	sed	317	[17]
SEDr	TAATGCTATATCTTATAGGG
SEEf	TAGATAAAGTTAAAACAAGC	see	170	[17]
SEEr	TAACTTACCGTGGACCCTTC
SEGf	TGCTATCGACACACTACAACC	seg	704	[18]
SEGr	CCAGATTCAAATGCAGAACC
SEHf	CGAAAGCAGAAGATTTACACG	seh	495	[18]
SEHr	GACCTTTACTTATTTCGCTGTC
SEIf	GACAACAAAACTGTCGAAACTG	sei	630	[18]
SEIr	CCATATTCTTTGCCTTTACCAG
SElJf	CAGCGATAGCAAAAATGAAACA	selj	426	[19]
SElJr	TCTAGCGGAACAACAGTTCTGA
SElMf	CCAATTGAAGACCACCAAAG	selm	517	[20]
SElMr	CTTGTCCTGTTCCAGTATCA
SElNf	ATTGTTCTACATAGCTGCAA	seln	682	[20]
SElNr	TTGAAAAAACTCTGCTCCCA
SElOf	AGTCAAGTGTAGACCCTATT	selo	534	[20]
SElOr	TATGCTCCGAATGAGAATGA
SElKf	ATGAATCTTATGATTTAATTTCAGAATCAA	selk	545	[21]
SElKr	ATTTATATCGTTTCTTTATAAGAAATATCG
SElQf	GGAAAATACACTTTATATTCACAGTTTCA	selq	539	[21]
SElQr	ATTTATTCAGTTTTCTCATATGAAATCTC
SElRf	AATGGCTCTAAAATTGATGG	selr	363	[22]
SElRr	TCTTGTACCGTAACCGTTTT
SElUf	AATGGCTCTAAAATTGATGG	selu	215	[22]
SElUr	ATTTGATTTCCATCATGCTC
TSST-1f	ATGGCAGCATCAGCTTGATA	tstH1	350	[17]
TSST-1r	TTTCCAATAACCACCCGTTT
16S rDNAf	ATA AGA CTG GGA TAA CTT CGG G	16SrDNA	500	[23]
16S rDNAr	CTT TGA GTT TCA ACC TTG CGG TCG

f: forward; r: reverse.