Figure 4.

The BM microenvironment as visualized in PMF with high grade reticulin and collagen fibrosis (PMF-3) accompanied by osteosclerosis. Absence of Nes⁺stromal cells in the dense fibrous tissue rimming the bone trabeculae while some Nes⁺ endothelial cells (red, arrows) of (a) arterioles or (b) sinusoids are still present. (c–f) Numerous CXCL12⁺ (green) stromal cells partially coexpressing (f) synemin (red, coexpression in yellow). (c, e) scattered Nes⁺ cytoplasmic fragments (arrows) suggestive of apoptotic cellular remnants.