Skip to main content
. 2015 Nov 30;2015:789414. doi: 10.1155/2015/789414

Figure 4.

Figure 4

Role of NFκB in IL-1β-induced hypoxic cell migration. Data are shown as means ± SEM. ∗ P < 0.05, statistically significant difference between IL-1β-treated hypoxic cells in the presence of BAY 11-7082 (IL-1β + BAY) and IL-1β-treated hypoxic cells in the absence of BAY 11-7082 (IL-1β). (a) Protein expression of NFκB was determined by Western blot analysis and a representative blot from three independent experiments is shown. Quantification of NFκB protein expression was achieved by chemiluminescence (n = 3). (b) The migration index was calculated as a fold change of relative fluorescence of cells migrating into the lower chamber normalized to that of the untreated samples (IL-1β) (n = 3). (c) CXCL8 mRNA and protein expression were analyzed by qRT-PCR and ELISA (n = 3).