Figure 3.

Characterization of endothelium-dependent relaxations to CJF in porcine coronary artery rings. Rings with endothelium were incubated with either indomethacin (10 μM), N^ω-nitro-L-arginine (L-NA, 10 μM), charybdotoxin (CTX, 100 nM) plus apamin (APA, 100 nM), or the PI3-kinase inhibitors wortmannin (30 nM) for 30 min before the contraction to U46619 and subsequent relaxation to CJF. The relaxation response is expressed as the percentage relaxation of the U46619-induced contraction. Results are shown as means ± SEM (n = 5). ^∗ P < 0.05, ^∗∗ P < 0.01, ^∗∗∗ P < 0.001, significant difference versus control.