Table 2.
Primary antibodies used for cell analysis.
| Primary antibodies | Species | Dilution | Manufacturer |
|---|---|---|---|
| α-smooth muscle actin | Rabbit | 1 : 100 | Spring Bioscience, Pleasanton, USA |
| c-kit | Rabbit | 1 : 500 | Dako, Hamburg, Germany |
| Fzd4 (clone CH3A4)∗ | Mouse | Undiluted | In-house (HJB) |
| Fzd4 PE (clone CH3A4)∗ | Mouse | 1 : 50 | Biolegend, San Diego, USA |
| GFAP | Rabbit | 1 : 400 | Dako, Hamburg, Germany |
| Nestin | Rabbit | 1 : 1000 | Abcam, Cambridge, UK |
| p75NTR | Rabbit | 1 : 500 | Promega, Madison, USA |
| p75NTR APC | Rabbit | 1 : 50 | Miltenyi, Bergisch Gladbach, Germany |
| Peripherin | Rabbit | 1 : 200 | Merck Millipore, Darmstadt, Germany |
∗Monoclonal mouse anti-human antibody CH3A4 against frizzled-4 was raised by immunization with the retinoblastoma cell line WERI-RB-1 and specificity for frizzled-4 was verified by the selective recognition of HEK-293 cells transfected with human frizzled-4. This molecule was clustered to CD344 at the HCDM workshop in Quebec, Canada (http://www.hcdm.org/).