Figure 6. Presence of insect chitinase gene in transgenic maize: Figure represents the outcome of PCR reactions carried out for detection of transgenic chitinase DNA in genomic DNA samples isolated from putatively transgenic plants.

(A). PCR products for plasmid pChi-SB, where ‘M’ is DNA marker 1kb; ‘ +ve’ is positive control using plasmid pChi-SB as template; ‘−ve’ is negative control using genomic DNA from an untransformed plant. Lanes 1–5 are genomic DNA samples from individual events of inbred resulting from biolistic gene gun transformation. (B). PCR product of partial-length bar gene. Lane ‘M’ is DNA marker; ‘ + ’ is positive control Chi-SB/Chitinase/bar plasmid, ‘−’ is negative control (non-transgenic line). Lane 1 to 5 are the inbreed lines of maize resulting from biolistic gene gun transformation.