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Representative fluorescent images of cells before (a) and after freezing (b) (green: live cells; red: dead cells; scale bar: 100 μm); (c) Survival rate at different processes; (d) Survival rate of cells frozen in various droplet volumes. *indicates p < 0.05 relative to 0.2 ul droplet; #indicates p < 0.05 relative to 5 ul droplet.
