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. 2015 Dec 14;5:17949. doi: 10.1038/srep17949

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Copyright © 2015, Macmillan Publishers Limited

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(a) PSTVd consists of five structural/functional domains. These are shown on the secondary structure of PSTVd, which was used for constructing various hairpin PSTVd (hpPSTVd). The yellow coloured region represents the CCR. The visible sequences are the regions that were used to make the constructs hpPSTVd:ΔTLE, hpPSTVd:TL, hpPSTVd:ΔP, hpPSTVd:Puppo, hpPSTVd:CCRuppo, hpPSTVd:CCRdopo and hpPSTVd-:257a. TL; terminal left, P; pathogenicity, CCR; conserved central region, V; variable region, TR; terminal right. The green coloured arrow indicates the anti-genomic direction used for hpPSTVd:257a construct. (b) Schematic view of all of the PSTVd regions used to produce inverted repeat constructs. The arrows indicate the PSTVd sequences used.