Table 1. List of transgenes, corresponding functional domains and PSTVd-sRNA hotspots and line number of the transgenic plants.
Name of Transgene | Functional domainof viroid | Hotspot of PSTVd-sRNAto express | Length of sequencein nt* | Promoter toexpress transgene | Line No. ofTransgenic plant |
---|---|---|---|---|---|
hpPSTVd:ΔTLE | Lacks terminal end of upper and lower TL | Almost the same to PSTVd | 340 | CoYMV | 24 |
32 | |||||
141 | |||||
hpPSTVd:TL | TL | P1 +M1 | 91 | CaMV-35S | 141 |
CoYMV | 85 | ||||
173 | |||||
hpPSTVd:ΔP | Lacks upper and lower P | Lacks P2 | 281 | CaMV-35S | 71b |
CoYMV | 82 | ||||
hpPSTVd:Puppo | Upper P | P2 | 26 | CaMV-35S | 54 |
122 | |||||
141 | |||||
161 | |||||
hpPSTVd:CCRuppo | Upper CCR | P3 + M2 | 49 | CaMV-35S | 65 |
114 | |||||
CoYMV | 12 | ||||
131 | |||||
hpPSTVd:CCRdopo | Lower CCR | P4 + M5 | 46 | CaMV-35S | 81 |
hpPSTVd:257a | Portion of lower CCR and V | M5 | 40 | CaMV-35S | 24 |
CoYMV | 25 | ||||
22 | |||||
60 |
*Length of the PSTVd sequence used to construct the IR.