Table 2.
H. pylori strains used in this study
| H. pylori strains | Strain # | Genotype/Description | Reference/source |
|---|---|---|---|
| G27 | Wild type | (Censini et al., 1996)/From Nina Salama | |
| mG27 | KO625 | G27, mouse-adapted | (Castillo et al., 2008) |
| G27-MA | G27, MDCK cells adapted | (Amieva et al., 2003) | |
| SS1 | Wild type, mouse adapted | (Lee et al., 1997) | |
| SS2000 | Wild type | (Thompson et al., 2004) | |
| PMSS1 | Wild type, Parent of strain SS1, not mouse adapted | (Arnold et al., 2011) | |
| 26695 | Wild type | (Tomb et al., 1997) | |
| J99 | Wild type | (Alm et al., 1999) | |
| G27 ΔcheZHP::KS | KO1269 | G27 ΔcheZHP::aphA3/sacB | This study |
| G27 ΔcheZHP | KO1315 | KO1269 ΔcheZHP (entire coding region deleted) | This study |
| G27 ΔcheZHP::cheZHP | KO1304 | KO1269 ΔcheZHP:: cheZHP (complement) | This study |
| G27 ΔcheZHP::cat | KO1325 | G27 ΔΔcheZHP::cat (retains coding potential for the first 13 and last 12 amino acids) | This study; allele originally published in (Terry et al., 2006) |
| G27 cheZHP Q193R | KO1307 | KO1269 ΔcheZHP::cheZHP Q193R | This study |
| G27 cheZHP D189N | KO1306 | KO1269 ΔcheZHP::cheZHP D189N | This study |
| G27 cheZHP ΔN39 | KO1313 | KO1269 ΔcheZHP:: cheZHP ΔN (deletion of amino acids 1–39) | This study |
| G27 cheZHP N-only | KO1273 | KO1269 ΔcheZHP:: cheZHP 1–39 (retains amino acids 1–39) | This study |
| G27 ΔcheZHP C12 | KO1300 | KO1269 ΔcheZHP:: ΔcheZHP C (deletion of C-terminal 12 amino acids) | This study |
| G27 cheZHP C-only | KO1312 | KO1269 ΔcheZHP:: cheZHP C-only (retains amino acids 241–253) | This study |
| G27 ΔcheW | KO851 | G27 ΔcheW::aphA3 | (Terry et al., 2005) |
| G27 ΔcheAY | KO857 | G27 ΔcheAY::cat (also called ΔcheA::cat) | This study; cheAY allele published in (Terry et al., 2005) |
| G27 ΔcheY | KO1250 | G27 ΔcheY::aphA3-sacB | This study |
| G27 ΔcheV1 | KO1277 | G27 ΔcheV1::cat | This study; cheV1 allele published in (Lowenthal, Simon, et al., 2009) |
| G27 ΔcheV2 | KO1278 | G27 ΔcheV2::cat | This study; cheV2 allele published in (Lowenthal, Simon, et al., 2009) |
| G27 ΔcheV3 | KO1279 | G27 ΔcheV3::cat | This study; cheV3 allele published in (Lowenthal, Simon, et al., 2009) |
| mG27 ΔtlpA | KO1002 | mG27 ΔtlpA | (Rader et al., 2011) |
| mG27 ΔtlpB | KO1004 | mG27 ΔtlpB | (Rader et al., 2011) |
| mG27 ΔtlpC | KO1005 | mG27 ΔtlpC::aphA3 | (Rader et al., 2011) |
| mG27 ΔtlpD | KO1006 | mG27 ΔtlpD::cat | (Rader et al., 2011) |
| mG27 ΔtlpB::kan-sac | KO1003 | mG27 ΔtlpB::aphA3-sacB | (Rader et al., 2011) |
| mG27 ΔtlpA ΔtlpD | KO1009 | KO1002 ΔtlpD::cat | This study |
| mG27 ΔtlpA ΔtlpB ΔtlpD | KO1015 | KO1009 ΔtlpB | This study |
| mG27 ΔtlpA ΔtlpB ΔtlpC ΔtlpD (Δtlps) | KO1021 | KO1015 ΔtlpC::aphA3 | This study |
| G27-MA ΔchePep | G27-MA ΔchePep::cat | (Howitt et al., 2011) | |
| G27-MA ChePep* | G27-MA ΔchePep::cat rdxA::chePep- ahpA3 | (Howitt et al., 2011) | |
| G27-MA ΔcheV1 ΔcheV2 | This study | ||
| H. pylori G27 flhA | KO1284 | G27 flhA::kan | (Rader et al., 2007). Gift of Karen Guillemin. |
| H. pylori G27 ΔflhF | KO1367 | G27 ΔflhF::cat (165 bp deletion) | This study. Allele provided by Nina Salama (Fred Hutchison Cancer Research Center, Seattle WA). |
| H. pylori G27 ΔflhG | KO1328 | G27 ΔflhG::cat (62 bp deletion) | This study. Allele provided by Nina Salama (Fred Hutchison Cancer Research Center, Seattle WA). |
| H. pylori G27 fliA | KO1285 | G27ΔfliA::kan | (Rader et al., 2007). Gift of Karen Guillemin. |
| G27-MA ΔfliF | G27 ΔfliF::cat | This study | |
| G27 ΔfliG | KO1063 | G27 ΔfliG::cat | This study |
| G27 ΔfliM | KO1060 | G27 ΔfliM::cat | (Lowenthal, Hill, et al., 2009) |
| G27 fliN (KO1061) | KO1061 | G27 ΔfliN::cat | (Lowenthal, Hill, et al., 2009) |
| G27 fliY (KO1062) | KO1062 | G27 ΔfliY::cat | (Lowenthal, Hill, et al., 2009) |
| G27 motB | KO489 | G27 motB::aphA3-sacB | (Ottemann and Lowenthal, 2002) |
| G27 Δhp0062 | KO1310 | G27_57/hp0062::cat | This study. Allele pro- vided by Nina Salama (Fred Hutchison Cancer Research Center, Seattle WA). |