Figure 1. Generation and characterization of scFv47.

(a) Screening of parental hybridoma IL13Rα2 cells mRNA for Vh and Vl using set Vh and Vl specific primers (Suppl. Table 1). (b) ELISA testing for phage binding enriched in three rounds of biopanning against rhIL13Rα2 protein and hFc as a negative control. (c) Competitive Assay. Plates coated with rhIL13Rα2 protein were treated with a panel of anti-IL13Rα2 mAbs that recognize non-overlapping epitopes with the parental mAb IL13Rα2 (clone 47)¹⁹. Binding of phages in the absence or presence of antibodies was analyzed via detection with anti-M13 phage antibody as described in the Material and Methods section. Each data point is an average of 3 independent replicates in all figures. Data presented as mean ± SEM. ***p < 0.001.