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. 2015 Dec 14;5:18133. doi: 10.1038/srep18133

Figure 5. IL13Rα2-specific Infectivity of Ad5FFscFv47-CMV-GFP.

Figure 5

(a) Flow cytometry analysis of IL13Rα2 expression in U251MG cells following knockdown with control shRNA (IL13Rα2+ U251MG) or IL13Rα2-specific shRNA (IL13Rα2KDU251MG) presented as percent of positive cells (flow charts) and median fluorescent intensity (MFI). (b) IL13Rα2-dependent infectivity of Ad5FFscFv47 -CMV-GFP demonstrated by differential expression of GFP in in IL13Rα2+ U251MG and IL13Rα2.KDU251MG cell lines. (c) Competitive binding assay. U251MG cells were pre-treated with anti-IL13Rα2 mAb as described in the Material and Methods section. Control and treated cells were then infected with Ad5scFv47-CMV-GFP virus. Cells were analyzed for GFP transgene expression 72 hours later by flow cytometry. Each data point is an average of 3 independent replicates. Mean ± SEM is plotted. ***p < 0.001.