Table 1. Kinetics of scFv47 binding to the human recombinant IL13Rα2.

	ka (1/Ms)	kd (1/s)	KD (M)	Rmax (RU)
scFv47	3.08e3 ± 16	2.63e-6 ± 1.8e-8	0.9 · 10-9	496

The estimation of kinetic parameters was performed as described in the ‘Materials and Methods’. The dissociation constant (K_D) of the complexes was determined as the ratio k_a/k_d. For quantitative analysis, 3 independent replicates were performed for each sample. Data are expressed as mean ± SEM. These data demonstrate that the affinity of scFv47 to rhIL13Rα2 is similar to parental mAb IL13Rα2 (clone 47) (1.39 · 10⁻9) as we have previously reported¹⁹.