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. 2015 Dec 14;5:18133. doi: 10.1038/srep18133

Table 1. Kinetics of scFv47 binding to the human recombinant IL13Rα2.

  ka (1/Ms) kd (1/s) KD (M) Rmax (RU)
scFv47 3.08e3 ± 16 2.63e-6 ± 1.8e-8 0.9 · 10-9 496

The estimation of kinetic parameters was performed as described in the ‘Materials and Methods’. The dissociation constant (KD) of the complexes was determined as the ratio ka/kd. For quantitative analysis, 3 independent replicates were performed for each sample. Data are expressed as mean ± SEM. These data demonstrate that the affinity of scFv47 to rhIL13Rα2 is similar to parental mAb IL13Rα2 (clone 47) (1.39 · 109) as we have previously reported19.