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. 2015 Dec 14;5:18136. doi: 10.1038/srep18136

Figure 2. hPSC culture on PVA-oligoVN hydrogels with optimal elasticity.

Figure 2

(A) Elasticity (storage modulus) is regulated by the crosslinking time on PVA-oligoVN hydrogels. (B) Morphology of hESCs (WA09) cultured on PVA-oligoVN hydrogels with several elasticities (PVA-1h-500, PVA-6h-500, PVA-12h-500, PVA-24h-500, and PVA-48h-500), Synthemax II dishes, and Matrigel at passage 1. The bar indicates 100 μm. (C) Attachment ratio of PSCs (blue bar, hESCs [WA09] and red bar, hiPSCs [HPS0077]) on PVA-oligoVN hydrogels with several elasticities, Synthemax II dishes, and Matrigel at passage 3. (D) Differentiation ratio of hPSCs (blue bar, hESCs [WA09] and red bar, hiPSCs [HPS0077]) on PVA-oligoVN hydrogels with several elasticities, Synthemax II dishes, and Matrigel at passage 3.