Table 1. Differentially expressed microRNAs in testis and sperm of Western vs. standard diet fed males.

Name	Read number		Fold change		Reference
	RNA-seq testis	RNA-seq testis	q-RT miRNA testis	q-RT miRNA sperm
Downregulated miRNAs
mmu-miR-183-5p	24	1.95*	1,8†	1,3	10,39
mmu-miR-182-5p	54	1.75**	2†	1,7†	39
Upregulated miRNAs
mmu-miR-124-3p	103	1.95*	ND	ND	40,41
mmu-miR-30e-5p	421	1.95*	1,1	1,2	40
mmu-miR-468-5p	78	1.65*	ND	ND	40,41
mmu-miR-29a-5p§	8161	1.5*	1,5†	1,3†	40,41
let-7i-3p§	41	1.6**	2	1	40
mmu-miR-340-5p	61	1.5**	1,5†	1,7†	26,42
mmu-miR-146a-5p	21	1.8*	ND	ND	41
mmu-miR-376b-5p	15	1.8*	ND	ND	40,41
mmu-miR-19b-3p§	16211	1.5*	1,8†	1,9†	40,41
mmu-miR-19a-3p§	3058	1.5*	1,8†	1,9†	40,41
mmu-miR-101b-3p	344	1.5**	ND	ND	27,41

Validation of selected differentially expressed miRNA (*p < 0.05, **p < 0.01) by quantitative reverse transcription polymerase reaction. Values are miRNA expression levels relative to internal controls (n = 10 for each group). SNORD61 was used for normalisation because of its stable expression in Western Diet vs. Standard Diet. The p value was calculated using the t test between western and standard diet samples ^†p < 0.05). The column “Reference” indicates the published articles showing an association between the indicated miRNA and the development of obesity and/or diabetes in different systems.