Figure 6. Flow cytometry assay of HepG2 apoptosis induced by Akbu-LAAO and exogenous H₂O₂ administrations.

The propidium iodide (PI) and FITC-labeled AnnexinV antibody were used for sample labeling reagents. HepG2 cells were incubated in the presence or absence of catalase for 1 h and treated with Akbu-LAAO or exogenous H₂O₂ for 24 h. Cells from each group were incubated with Annexin V-FITC and PI, and immediately subjected to flow cytometry assay. Triplicate experiments were performed for each group. *, ** and *** denote P < 0.05, 0.01 and 0.001, respectively.