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. 2015 Dec 14;5:42. doi: 10.1186/s13395-015-0067-1

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© Pawlikowski et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Satellite cell contribution to myofibers is age dependent and varies in different muscles. a–b Non-linear regression trend lines for percentages of tdTomato-expressing myofibers through experimental time points from the EOM and diaphragm (a) and EDL, soleus, and gastrocnemius (b). c–e Quantification of myofiber tdTomato-expression of hindlimb, EOM, and diaphragm muscles with recombination induced at 4 (c), 12 (d), or 27 weeks (e). Quantification of tdTomato was evaluated at four relative levels of expression: +++ very high tdTomato intensity (see example myofiber “1” in Fig. 3c, 12 weeks), + intermediate tdTomato intensity (see example myofiber “2” in Fig. 3c, 12 weeks), +/− very low tdTomato intensity myofibers and scored as negative (see example myofiber “3” in Fig. 3c, 12 weeks), and – fibers do tdTomato intensity indistinguishable from background (see example myofiber “4” in Fig. 3c, 12 weeks)