Fig. 8.

Satellite cells fuse into uninjured myofibers either with our without an intervening cell division. a–c Representative images show EdU+ satellite cells and EdU-labeled nuclei within myofibers. a Myofiber showing an EdU+ satellite cell (arrow) and an EdU+ differentiated myonuclei (arrowhead). b Images show a myofiber with two satellite cells that are EdU+. c A myofiber with two EdU+ myonuclei within the myofiber. For a–c, DAPI (blue), tdTomato (red), EdU (green), and Pax7 (white). Scale bar is 20 μm. d Quantification showing the percentages of satellite cells on dtTomato+ (Tom pos) myofibers versus dtTomato− myofibers (Tom neg) that were labeled with EdU. e Bar graph showing the percentages of myofibers that have at least one EdU+ associated satellite cell. f Quantification showing the percentages of myofibers that have at least one EdU+ nuclei within the myofiber. g Quantification of the satellite cell number present on dtTomato+ myofibers versus dtTomato− myofibers (*p ≤ 0.01). h Images of TA muscle sections show an EdU+, tomato+ SC. tdTomato (red), EdU (green), laminin (white), and DAPI (blue). Scale bar is 40 μm. For bar graphs, ns denotes not significant. Significance was determined by Student’s t test. N = 3. For all graphs, the mean with the standard error of the mean is plotted