Table 1.
Quantification of tdTomato recombination
| Percentage of tdTomato+ SCs | 8 weeks | 12 weeks | 27 weeks | All time points |
|---|---|---|---|---|
| EDL | 100.0 | 95.2 | 100.0 | 98.7 |
| Sol | 94.7 | 100.0 | 94.4 | 97.1 |
| EOM | 95.0 | 100.0 | 96.6 | 97.1 |
| Gastroc | 100.0 | 97.1 | 100.0 | 99.4 |
| TA | 96.5 | 97.4 | 100.0 | 97.6 |
| Diaphragm | 100.0 | 100.0 | 96.7 | 99.0 |
Cross-sections of indicated muscle with recombination induced at 8, 12, or 27 weeks were stained for Pax7, laminin, and counterstained with DAPI to mark nuclei. The number of tdTomato-expressing Pax7+ satellite cells was counted and divided by the total number of Pax7+ satellite cells. Between 20 and 80 satellite cells were counted for each experimental time point per muscle. A total of 652 satellite cells were counted. Three to six biological replicates were analyzed per time point