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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: J Biomol Screen. 2011 Jun 21;16(8):836–844. doi: 10.1177/1087057111411088

FIG. 4.

FIG. 4

Ultra-high-throughput screening (uHTS) format assay validation. The time-resolved fluorescence resonance energy transfer (TR-FRET) assay that monitors GTP cyclohydrolase 1 (GTPCH-1)/GTP cyclohydrolase feedback regulatory protein (GFRP) interaction was validated for uHTS using the Library of Pharmacologically Active Compounds (LOPAC) library in four replicates. Signal-to-background (S/B) ratios (A) and Z′ factors (B) were evaluated for four plates. (C) The percentage of control is calculated as defined in the Materials and Methods section and plotted against compound ID. The potential positives are defined by the compound with % inhibition >30. Representative dose-response curves of the potential positive compounds determined by the TR-FRET assay are shown in panels D, E, and F.