Table 1.
Effect of ouabain on intracellular Na+ and K+ content in human (HASMC, HUVEC) and rat (RASMC, RAEC) cells
Type of cells | Ouabain, μM | Intracellular Na+ (nmol/mg protein) |
Intracellular K+ (nmol/mg protein) |
---|---|---|---|
HASMC | 0 | 64±20 | 1211±60 |
3 | 701±38* | 108±11* | |
3000 | 766±90* | 132±38* | |
| |||
HUVEC | 0 | 114±24 | 1311±89 |
3 | 978±53* | 133±20* | |
3000 | 1016±97* | 129±8* | |
| |||
RASMC | 0 | 111±23 | 1211±93 |
3 | 178±11 | 1097±68 | |
3000 | 876±71* | 156±27* | |
| |||
RAEC | 0 | 56±8 | 722±59 |
3 | 69±12 | 693±82 | |
3000 | 766±54* | 86±21* |
Cells were incubated in DMEM medium with or without ouabain for 6 h. Means ± S.E. from experiments performed in triplicates are shown.
p<0.001 compared to ouabain-free medium.