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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Mol Biol. 2016;1382:467–482. doi: 10.1007/978-1-4939-3271-9_31

Figure 4. Workflow for T cell proliferation assay.

Figure 4

Purify splenocytes, remove RBCs, label with CFSE dye and culture with tumor lysate followed by processing for flow cytometric analysis. Dot plots show gating strategy for CD8 T cells. Histograms show representative images of CFSE stains in unstimulated and stimulated CD8 T cells.