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. 2015 Dec 14;6:627. doi: 10.3389/fimmu.2015.00627

Figure 2.

Figure 2

HDI selectively inhibits mRNA expression in B cells induced to undergo CSR and plasma cell differentiation. B cells were cultured with LPS plus IL-4 in the presence of HDI (VPA, 500 μM) or nil for 60 h before preparation of RNA for mRNA-Seq. (A) Scatter plots of average mRNA expression levels in three independent experiments (reads per kilobase per million mapped reads, RPKMs), in LPS plus IL-4-stimulated B cells treated with VPA versus those in LPS plus IL-4-stimulated B cells treated with nil. Each plot corresponds to one individual mRNA expression level. (B) Bar graphs depict the average folds of changes of mRNA expression levels (average RPKMs from three independent experiments) in LPS plus IL-4-stimulated B cells treated with HDI, as compared to those in B cells treated with nil. Only the mRNAs at average RPKM >20 in LPS plus IL-4-stimulated B cells treated with nil are included. Aicda, Prdm1, and Xbp1 mRNAs and the mRNAs that were increase or decreased or increased in average by more than twofold are indicated. p values, paired t-test.